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<“·Ò†’ªÅéU-Ç<{&®Ò3Ä �ðNë�GVÚèÞW(7 » ôùþ=›5m"p¹Âp‹˜—	£	¬	¸	Â	Í	û	p
ÌÏ\Ï×Å×:M~
Gé„—ó•žðÝ‚Ï<Î2 Z œ ³èW,'epŠ ºÄae1W]è		G	X	a	¼	
v
'á_•BßÌ×PÑ„ÁÅú%�Ü÷K] 0 ˆ `ãTæsÖyˆúè3=]" | ¢ Ó ñ ^{¹Q�@$þª�	§	Æ	Ò	[
6ï&3¥ÐüEzöAž y « í‚Ymß^¹X
<œžMZ‡ë  ã šÀÔ|°¥ØJ°¤
^¡°oÀ"†¸]u€’Nã  ðatœœ	±	/
s›§7 ÷ nÉÕÇ
Î×éš¢ØL®¹p  Dÿbºþ	
ŽÖŸf›MhóÁ ê Œ… 6ž:¸–	«	f
;’‘T;7V­k" › ª ²ìzâÞÃå#0		W	`	¦	»	Å	Ñ	5xîù2AÞƒ€�êöJ â ™¿ïÓÈþI›	°	n!eÍ\g¡! ±5œ�9Âä		V	_	š	¯	Ð	:‘ø1	
Z[ÿ U‚«¬I  .*  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                           0                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                2                	                            	                                                         
                         	                                                      %            	                  	      H            	                                                                                    #                      7         )   $      "   .      !   ™   G         
             Z                                                                                                                                                                      0                                                                          
                                                                                                                                                                                                                                                                                                                            2      	          	                     
         	                    %    	      	  H    	                            #        7   ) $  " .  ! ™ G   
     Z   iú  k  k  kN  oœ  tP  z”ñt"óŒD›'œ�þWÍ	×éÌÞ„›RQl½Ô 	h	W~
­µZú:
5ñûÑ— Ôÿ÷	9x9x*	r	  IrÊ\ª³´@ô»M‡*	r	¥	ô	ú	¸
Ù
ú
&Nu¼Åä^P´Ü38yë'ò”—Èa;O Á_RQRQRQˆ <lšíTêã 	X	×o×  0Ã!ï…Ã!ï¼ e¼ V¹D7 DÔS’AŸ¹F	— õ½—˜a”ÃFŸý,
o7ÂR²¡|V !4�
 æ í •MÚ1Ä˜ä5"Y5'7w Q^„ˆÌ
[*{ð>Úïà�
�ËÈ
 1ñ´nÙ H)>¼és“ �ø…öœö   :‘T˜ õ/
³ ˜ ç –�ÛÅõöQ^
>} ˜ õ»uœi1± ˆ/	w	�Â– §—ôw»	AÞöJÅ„ž Ù H1ŒY�T
)I>° ˜ · æ í •¯MÚ1Ä˜}	[	õöÞä5Ú"€©|YÍÏ,h¾&AÂŸÅ| â WãYNp;õ0ª	W	Ò	õ	/
yún’!¸\ÝÂ%f ÙésìŠŒ¹'B
 ½ é˜ý¨†L]á<s8¦-O¢¥
„ˆÌð› ²Ã	`	ƒ€qìmTÉ a-«„ˆÌìJÄeðÅš| ªÒ	 ˜ ç –��ÛøÅ…õ»öœhÅ#Œ¼R| ãªÒ	/
yú“BŸº
5  ½ Á 7{Ìç¸&\ý`·PV†	/	d	w	& ^&*LY]náü(<O8uËð Ä0| YNªÒ	Ý1ed/	w	v¼¾}=HžªÇØÊò ºoÆÝRf±ÄÝØ
¸l¢	·	Á	Ì	[–ï �ÍËÈ
 	b	6T%ÆÑÕÙK¢Èï1	y	1Â2 ihÝû1”T�o	7ŸEÂöÞ¢R! 'ùqÅ#w
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�¿Sm¥+�ïBŒm¦ z2°°
C]½ ý†L]á<8Æ (f˜éâÅÚ1í‚Ýô	Á
Ñ  Æ éÂ  h¾Ï&,AÂŸx°NDÃÅ›ôëd“ö!hæ
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<UUID>00ace6f2-2d61-4f0d-b244-a862a0f96b52</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGL4.27[luc2P/minP/Hygro]</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter for measuring the activity of transcriptional response elements using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="5:0:0">2006.9.26</Created>
<LastModified UTC="1:59:59">2021.3.8</LastModified>
<AccessionNumber>DQ904459</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
<Organism>Mammalian Cells</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
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<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter for measuring the activity of transcriptional response elements using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="5:0:0">2006.9.26</Created>
<LastModified UTC="1:59:56">2021.3.8</LastModified>
<AccessionNumber>DQ904459</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
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<SequenceClass>UNA</SequenceClass>
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<UUID>00ace6f2-2d61-4f0d-b244-a862a0f96b52</UUID>
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<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter for measuring the activity of transcriptional response elements using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="5:0:0">2006.9.26</Created>
<LastModified UTC="1:59:49">2021.3.8</LastModified>
<AccessionNumber>DQ904459</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
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<SequenceClass>UNA</SequenceClass>
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<UUID>00ace6f2-2d61-4f0d-b244-a862a0f96b52</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGL4.27[luc2P/minP/Hygro]</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter for measuring the activity of transcriptional response elements using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="5:0:0">2006.9.26</Created>
<LastModified UTC="1:59:24">2021.3.8</LastModified>
<AccessionNumber>DQ904459</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
<Organism>Mammalian Cells</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
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  ‹     ÿ BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ‰<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="0" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2266-2623" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="HygR" directionality="1" translationMW="38444.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2654-3691" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;hygromycin B phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEVGRV*"/></Q></Feature><Feature recentID="2" name="minP" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="63-94" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;minimal TATA-box promoter with low basal activity&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5843-5891" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3715-3763" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="pause site" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5905-5996" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase II transcriptional pause signal from the human&amp;nbsp;Î±2 globin gene&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="hPEST" directionality="1" translationMW="4153.63" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1902" maxFusedRunOn="1902"><Segment range="1780-1899" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;PEST degradation sequence from mouse ornithine decarboxylase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1951-2072" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4090-4678" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5669" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4878-5669" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5670-5738" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="10" name="luciferase" directionality="1" translationMW="60644.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1902" maxFusedRunOn="1902"><Segment range="127-1776" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;synthetic &lt;i&gt;luc2&lt;/i&gt; version of the luciferase gene&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"/></Q></Feature><Feature recentID="11" name="PDL1" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="42-47" color="#ffffff" type="standard"/></Feature></Features>
  Ý<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="15" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RVprimer3" sequence="CTAGCAAAATAGGCTGTCCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5944-5963" boundStrand="0" annealedBases="CTAGCAAAATAGGCTGTCCC" meltingTemperature="54"><Component hybridizedRange="5944-5963" bases="CTAGCAAAATAGGCTGTCCC"/></BindingSite><BindingSite simplified="1" location="5944-5963" boundStrand="0" annealedBases="CTAGCAAAATAGGCTGTCCC" meltingTemperature="54"><Component hybridizedRange="5944-5963" bases="CTAGCAAAATAGGCTGTCCC"/></BindingSite></Primer><Primer recentID="1" name="RVprimer4" sequence="GACGATAGTCATGCCCCGCG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="3829-3848" boundStrand="1" annealedBases="GACGATAGTCATGCCCCGCG" meltingTemperature="62"><Component hybridizedRange="3829-3848" bases="GACGATAGTCATGCCCCGCG"/></BindingSite><BindingSite simplified="1" location="3829-3848" boundStrand="1" annealedBases="GACGATAGTCATGCCCCGCG" meltingTemperature="62"><Component hybridizedRange="3829-3848" bases="GACGATAGTCATGCCCCGCG"/></BindingSite></Primer></Primers>
  ä<Notes>
<UUID>df448c4d-71cf-4d8f-aea0-80a76e7ca13d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGL4.27-PDL1(human)-mut-miniP-Fluc-SV40-Hygro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Vector with a minimal promoter for measuring the activity of transcriptional response elements using destabilized luciferase.&lt;/body>&lt;/html></Description>
<Created UTC="5:0:0">2006.9.26</Created>
<LastModified UTC="4:53:0">2025.9.3</LastModified>
<AccessionNumber>DQ904459</AccessionNumber>
<CreatedBy>Promega</CreatedBy>
<Organism>Mammalian Cells</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y      K         
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   )<?xml version="1.0"?><CustomEnzymeSets/>
